Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25927||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)3800
MutationThis myc-RapR-FAK-YM plasmid contains FAK mutations Y180A and M183A. (The sequence data uploaded to addgene is based on wt).
Entrez GenePtk2 (a.k.a. FADK 1, FAK, FRNK, Fadk, p125FAK)
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CMV-Fw (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:myc-Rapr-FAK-YM was a gift from Klaus Hahn (Addgene plasmid # 25927 ; http://n2t.net/addgene:25927 ; RRID:Addgene_25927)
For your References section:Engineered allosteric activation of kinases in living cells. Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM. Nat Biotechnol. 2010 Jun 27. ():. 10.1038/nbt.1639 PubMed 20581846
Map uploaded by the depositor.