|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27803||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepEGFP C1
- Backbone size w/o insert (bp) 3984
Vector typeMammalian Expression ; FRET standard
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCerulean Traf Venus
Insert Size (bp)2138
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe 1 (not destroyed)
- 3′ cloning site Bam HI (not destroyed)
- 5′ sequencing primer CCAAAATCAACGGGACTTTCC
- 3′ sequencing primer CAGGTTCAGGGGGAGGTGTGG (Common Sequencing Primers)
Terms and Licenses
Contains TRAF2 nucleotides 874-1561 from NM_021138.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CTV was a gift from Steven Vogel (Addgene plasmid # 27803)
For your References section:Energy migration alters the fluorescence lifetime of Cerulean: implications for fluorescence lifetime imaging Forster resonance energy transfer measurements. Koushik SV, Vogel SS. J Biomed Opt. . 13(3):031204. 10.1117/1.2940367 PubMed 18601528
Map uploaded by the depositor.