pEBG-NHERF2
(Plasmid
#28292)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 28292 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEBG
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Backbone manufacturerBruce Mayer, David Baltimore
- Backbone size w/o insert (bp) 6034
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNHERF2
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SpeciesH. sapiens (human)
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Entrez GeneSLC9A3R2 (a.k.a. E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBG-NHERF2 was a gift from Maria-Magdalena Georgescu (Addgene plasmid # 28292 ; http://n2t.net/addgene:28292 ; RRID:Addgene_28292) -
For your References section:
NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM. Mol Cell Biol. 2007 Apr . 27(7):2527-37. 10.1128/MCB.01372-06 PubMed 17242191