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AAV-Flex-rev-oChIEF-tdTomato Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid AAV-Flex-rev-oChIEF-tdTomato with the following enzymes: SmaI and PvuII-HF single digests We expected and saw the following band sizes for this digest: (1) SmaI - 4.4kb, 2.6kb and 0.4kb (2) PvuII - 4.9kb and 2.5kb

Addgene diagnostic digest 2

This gel depicts the NEB 1kb ladder and a digestion of the plasmid AAV-Flex-rev-oChIEF-tdTomato with the following enzyme: SnaBI single digest. We expected and saw the following band sizes for this digest: 4.3kb, 2.8kb and 0.2kb