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pcDNA mouse NHERF-1 (1-325)
(Plasmid #32706)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 32706 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5600
  • Modifications to backbone
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    Deleted last 30 AA putative Ezrin binding site
  • GenBank ID
  • Entrez Gene
    Slc9a3r1 (a.k.a. EBP-50, NHE-RF, NHERF-1, NHERF1)
  • Promoter CMV
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site v=Hind3 i=Xho1 filled 7 blunted (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA mouse NHERF-1 (1-325) was a gift from Edward Weinman (Addgene plasmid # 32706 ; ; RRID:Addgene_32706)
  • For your References section:

    Ezrin binding domain-deficient NHERF attenuates cAMP-mediated inhibition of Na(+)/H(+) exchange in OK cells. Weinman EJ, Steplock D, Wade JB, Shenolikar S. Am J Physiol Renal Physiol. 2001 Aug;281(2):F374-80. 10.1152/ajprenal.2001.281.2.F374 PubMed 11457730