pcDNA mouse NHERF-1 (1-325)
(Plasmid #32706)

• Depositing Lab: Edward Weinman
• Publication: Weinman et al Am J Physiol Renal Physiol. 2001 Aug;281(2):F374-80.

Backbone

• Vector backbone: pcDNA3.1/Hygro(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Alt name: NHERF-1
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Mutation: Deleted last 30 AA putative Ezrin binding site
• GenBank ID: U74079
• Entrez Gene: Nherf1 (a.k.a. EBP-50, NHE-RF, NHERF-1, Slc9a3r1)
• Promoter: CMV
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: v=Hind3 i=Xho1 filled 7 blunted (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry