pLJD-KLF4-ER
(Plasmid #34596)

• Depositing Lab: Michael Ruppert
• Publication: Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.

Backbone

• Vector backbone: pLJD
• Backbone manufacturer: LT Chow/UAB
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: use Kan at 50ug/ml
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KLF4
• Alt name: GKLF
• Alt name: EZF
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• GenBank ID: AF105036
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)
• Promoter: LTR
• Tags / Fusion Proteins:
  • Myc epitope tag (N terminal on insert)
  • estrogen receptor fusion (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBluescriptSK
• 3′ sequencing primer: use ER internal primers

Resource Information

• A portion of this plasmid was derived from a plasmid made by: insert transferred from pBABE-puro KLF4-ER, see Oncogene 2005 Foster KW et al. PMID:15674344

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

see information provided for pLJD vector