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(Plasmid #34662)


Item Catalog # Description Quantity Price (USD)
Plasmid 34662 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript KS(+)
  • Backbone size w/o insert (bp) 2958
  • Vector type
    Xenopus oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Top 10 F' E. coli
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    BK channel
  • Alt name
    Lablife ID:L1089.P202
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    slo-1 (a.k.a. CELE_Y51A2D.19)
  • Tags / Fusion Proteins
    • Xenopus beta-globulin 5' UTR (N terminal on insert)
    • Xenopus beta-globulin 3' UTR (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The backbone plasmid containing insert slo-1(A2;B0;C1) was obtained from Zhao-Wen Wang (University of Connecticut)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mg182 was a gift from Miriam Goodman (Addgene plasmid # 34662 ; ; RRID:Addgene_34662)
  • For your References section:

    Alternatively spliced domains interact to regulate BK potassium channel gating. Johnson BE, Glauser DA, Dan-Glauser ES, Halling DB, Aldrich RW, Goodman MB. Proc Natl Acad Sci U S A. 2011 Nov 2. 10.1073/pnas.1116795108 PubMed 22049343