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pET28a-SpyTagMBP Citations (7)

Originally described in: Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24.
PubMed Journal

Articles Citing pET28a-SpyTagMBP

Articles
Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. PubMed

Associated Plasmids

Engineering a Rugged Nanoscaffold to Enhance Plug-and-Display Vaccination. Bruun TUJ, Andersson AC, Draper SJ, Howarth M. ACS Nano. 2018 Jul 20. doi: 10.1021/acsnano.8b02805. PubMed

Associated Plasmids

Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Methods Enzymol. 2019;617:443-461. doi: 10.1016/bs.mie.2018.12.010. Epub 2019 Jan 25. PubMed
Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity. Izore T, Candace Ho YT, Kaczmarski JA, Gavriilidou A, Chow KH, Steer DL, Goode RJA, Schittenhelm RB, Tailhades J, Tosin M, Challis GL, Krenske EH, Ziemert N, Jackson CJ, Cryle MJ. Nat Commun. 2021 May 4;12(1):2511. doi: 10.1038/s41467-021-22623-0. PubMed
N-Terminal Modification of Gly-His-Tagged Proteins with Azidogluconolactone. Brune KD, Lieknina I, Sutov G, Morris AR, Jovicevic D, Kalnins G, Kazaks A, Kluga R, Kastaljana S, Zajakina A, Jansons J, Skrastina D, Spunde K, Cohen AA, Bjorkman PJ, Morris HR, Suna E, Tars K. Chembiochem. 2021 Nov 16;22(22):3199-3207. doi: 10.1002/cbic.202100381. Epub 2021 Oct 6. PubMed
Proactive vaccination using multiviral Quartet Nanocages to elicit broad anti-coronavirus responses. Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP, Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, Howarth MR. Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9. PubMed

Associated Plasmids

Development of a Two-Component Nanoparticle Vaccine Displaying an HIV-1 Envelope Glycoprotein that Elicits Tier 2 Neutralising Antibodies. Malebo K, Woodward J, Ximba P, Mkhize Q, Cingo S, Moyo-Gwete T, Moore PL, Williamson AL, Chapman R. Vaccines (Basel). 2024 Sep 18;12(9):1063. doi: 10.3390/vaccines12091063. PubMed

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