pcDNA5FRT PUR mGSK3AWT
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35159||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 5100
Modifications to backboneA mammalian expression plasmid containing a single FRT recombination site was created by splicing the CMV promoter, multiple cloning site, polyA signal, and puromycin-resistance cassette from pPUR (Clontech) into the BspEI and Bst11071 sites of pcDNA5/FRT (Invitrogen) to yield pcDNA5/FRT_puro.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneGsk3a (a.k.a. 2700086H06Rik)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Please note: this plasmid carries a serine (not alanine) at amino acid position 21. The S21A mutation shown on author's map indicates where the S21 is, for scientists that wish to mutate it.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA5FRT PUR mGSK3AWT was a gift from Jim Woodgett (Addgene plasmid # 35159 ; http://n2t.net/addgene:35159 ; RRID:Addgene_35159)
For your References section:Functional redundancy of GSK-3alpha and GSK-3beta in Wnt/beta-catenin signaling shown by using an allelic series of embryonic stem cell lines. Doble BW, Patel S, Wood GA, Kockeritz LK, Woodgett JR. Dev Cell. 2007 Jun;12(6):957-71. 10.1016/j.devcel.2007.04.001 PubMed 17543867
Map uploaded by the depositor.