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pcDNA5FRT PUR mGSK3A K148A
(Plasmid #35158)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35158 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 35158-D50 50 μg of DNA in Tris buffer $495 *
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DNA 35158-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pcDNA5/FRT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Modifications to backbone
    A mammalian expression plasmid containing a single FRT recombination site was created by splicing the CMV promoter, multiple cloning site, polyA signal, and puromycin-resistance cassette from pPUR (Clontech) into the BspEI and Bst11071 sites of pcDNA5/FRT (Invitrogen) to yield pcDNA5/FRT_puro.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSK-3α
  • Alt name
    Gsk3a
  • Species
    M. musculus (mouse)
  • Mutation
    K148A (dominant negative)
  • Entrez Gene
    Gsk3a (a.k.a. 2700086H06Rik)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S21A mutation shown on author's map indicates where the S21 is, for scientists that wish to mutate it. This plasmid carries a serine (not alanine) at amino acid position 21.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5FRT PUR mGSK3A K148A was a gift from Jim Woodgett (Addgene plasmid # 35158 ; http://n2t.net/addgene:35158 ; RRID:Addgene_35158)

  • For your References section:

    Functional redundancy of GSK-3alpha and GSK-3beta in Wnt/beta-catenin signaling shown by using an allelic series of embryonic stem cell lines. Doble BW, Patel S, Wood GA, Kockeritz LK, Woodgett JR. Dev Cell. 2007 Jun;12(6):957-71. 10.1016/j.devcel.2007.04.001 PubMed 17543867
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