|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37167||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUX2996 and pUX2410
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Growth Strain(s)E. Coli UQ5853
Growth instructionsCopy number: pUX2410 High / pUX2996 Low
Gene/Insert namecoxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag
- Cloning method Unknown
- 5′ sequencing primer pEXT20-F1
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan.
For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ5875 was a gift from Gary Roberts (Addgene plasmid # 37167)