|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37829||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7524
Vector typeMammalian Expression, Adenoviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human)
Entrez GeneMAPRE1 (a.k.a. EB1)
- Promoter CMV
/ Fusion Protein
- mCherry (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pShuttle-mCherry-EB1-C was a gift from Torsten Wittmann (Addgene plasmid # 37829)
For your References section:EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Gierke S, Wittmann T. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. 10.1016/j.cub.2012.02.069 PubMed 22483942
Generated by Addgene from full sequence supplied by depositor.