|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38149||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KSII(+)
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 6461
Vector typeWorm Expression ; recombineering
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt namerescue construct for unc-119(ed3) mutants
SpeciesC. elegans (nematode)
Entrez GeneCbr-unc-119 (a.k.a. CBG18291)
- Promoter unc-119
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3’ UTR of the Cbr-unc-119 gene.
The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPK719 was a gift from Patricia Kuwabara (Addgene plasmid # 38149 ; http://n2t.net/addgene:38149 ; RRID:Addgene_38149)