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(Plasmid #38149)


Item Catalog # Description Quantity Price (USD)
Plasmid 38149 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript KSII(+)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 6461
  • Vector type
    Worm Expression ; recombineering

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    rescue construct for unc-119(ed3) mutants
  • Alt name
  • Species
    C. elegans (nematode)
  • Entrez Gene
    Cbr-unc-119 (a.k.a. CBG18291)
  • Promoter unc-119

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3’ UTR of the Cbr-unc-119 gene.

The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPK719 was a gift from Patricia Kuwabara (Addgene plasmid # 38149 ; ; RRID:Addgene_38149)