pCAG:Kaede
(Plasmid #39261)

• Depositing Lab: Anna-Katerina Hadjantonakis
• Publication: Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

Backbone

• Vector backbone: pCS2-Kaede
• Modifications to backbone: CAG::Kaede was generated by PCR of Kaede from pCS2-Kaede [29] using oligos Kaede- 5F-EcoR1: CCGGAATTCCGG ATGGTGAGTCTGATTAAA CCAGAAATGAAG and Kaede-3R_EcoR1: CCGGAATTCCG GTTACTTGACGTTGTCCGGCAATCCAGAATG. The resulting PCR products for each vector were cut with EcoRI and cloned into pCAGGS [26].
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Kaede
• Alt name: CAG: Kaede
• Species: M. musculus (mouse)
• Promoter: pCAGGS

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CCGGAATTCCGG ATGGTGAGTCTGATTAAA CCAGAAATGAAG
• 3′ sequencing primer: CCGGAATTCCG GTTACTTGACGTTGTCCGGCAATCCAGAATG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry