pAM3110
(Plasmid #40247)

• Depositing Lab: Susan Golden
• Publication: Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13.

Backbone

• Vector backbone: pAM2314
• Total vector size (bp): 8300
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: attP Site
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 2500
• Promoter: n/a

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank File
  • Strain Database Information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

our engineered NS1 Gateway “donor vector”
the attP region of pDONR221 was amplified with primers H3140 and H3141, incorporating MluI and XhoI sites respectively and cloned into the NS1 vector pAM2314. the attP amplified region contains the upstream and downstream M13F and M13R annealing sites for sequencing inserts