|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40247||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8300
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
Gene/Insert nameattP Site
SpeciesSynechococcus elongatus PCC 7942
Insert Size (bp)2500
- Promoter n/a
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
our engineered NS1 Gateway “donor vector”
the attP region of pDONR221 was amplified with primers H3140 and H3141, incorporating MluI and XhoI sites respectively and cloned into the NS1 vector pAM2314. the attP amplified region contains the upstream and downstream M13F and M13R annealing sites for sequencing inserts
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM3110 was a gift from Susan Golden (Addgene plasmid # 40247 ; http://n2t.net/addgene:40247 ; RRID:Addgene_40247)
For your References section:Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes. Ditty JL, Canales SR, Anderson BE, Williams SB, Golden SS. Microbiology. 2005 Aug;151(Pt 8):2605-13. 10.1099/mic.0.28030-0 PubMed 16079339