|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40351||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid A. Williams (PMID: 10961859)
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt namenuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
SpeciesM. musculus (mouse)
Entrez GeneNFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
/ Fusion Protein
- IRES eGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
Terms and Licenses
The NFAT2-expressing retroviruses were constructed by inserting blunt-ended NFAT2 cDNA from pSH160C into the retroviral vector pMIEG3 via the blunt-ended XhoI site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIEG3-NFAT2 was a gift from Alexander Dent (Addgene plasmid # 40351 ; http://n2t.net/addgene:40351 ; RRID:Addgene_40351)
For your References section:Regulation of Th2 cytokine expression in NKT cells: unconventional use of Stat6, GATA-3, and NFAT2. Wang ZY, Kusam S, Munugalavadla V, Kapur R, Brutkiewicz RR, Dent AL. J Immunol. 2006 Jan 15;176(2):880-8. PubMed 16393972