pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]
(Plasmid #40806)

• Depositing Lab: Natalie Ahn
• Publication: Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Backbone

• Vector backbone: pMM9 from pMB1
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6900
• Modifications to backbone: pMB1: Blair et al. PNAS 77,3504 (1980)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418) ; G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAP2K2
• Alt name: MKK2
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: delta(48-55) + S222D + S226D
• GenBank ID: NM_030662
• Entrez Gene: MAP2K2 (a.k.a. CFC4, MAPKK2, MEK2, MKK2, PRKMK2)
• Promoter: MSV-LTR
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: MSV-LTR

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Kun-liang Guan provided gene, Dr. George Vande Woude provided vector backbone

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry