Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

H9 pMalLP
(Plasmid #41811)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41811 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMalLP
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 7000
  • Modifications to backbone
    The vector is a variant of pMalp2x, with a modified MCS and an N-terminal 3C protease cleavage site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H9 Super-2
  • Alt name
    H9
  • Alt name
    Super-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    429
  • Promoter pTac
  • Tags / Fusion Proteins
    • Maltose Binding Protein (N terminal on backbone)
    • 3C protease site (N terminal on backbone)
    • 6x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Mal-E
  • 3′ sequencing primer M13-65
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This is an e. coli periplasmic expression vector.

H9 has the following mutations compared to wildtype IL-2: L80F, R81F, L85V, I86V, I92F

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H9 pMalLP was a gift from Chris Garcia (Addgene plasmid # 41811 ; http://n2t.net/addgene:41811 ; RRID:Addgene_41811)
  • For your References section:

    Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'. Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC. Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975. 10.1038/nature10975 PubMed 22446627