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Addgene

alpha cat in pGEX-4T2
(Plasmid #42611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42611 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-4T2
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    catalytic domain of diacylglycerol kinase alpha
  • Alt name
    alpha cat
  • Alt name
    DGKA
  • Species
    S. scrofa
  • Insert Size (bp)
    1200
  • Mutation
    deleted amino acids 1-332, as well as the C-terminal cysteine
  • GenBank ID
    NM_214032.2
  • Entrez Gene
    DGKA (a.k.a. DAGK)
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • thrombin site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha cat in pGEX-4T2 was a gift from Daniel Raben (Addgene plasmid # 42611)
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