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Addgene

pBS-KSTbx6w32
(Plasmid #42662)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42662 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript II-KS(-)
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4150
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tbx6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1150
  • Entrez Gene
    Tbx6 (a.k.a. rv)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Received from Lee Silver.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Agulnik et al., 1996. Genetics 144:249-254.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-KSTbx6w32 was a gift from Virginia Papaioannou (Addgene plasmid # 42662)

  • For your References section:

    Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation. Chapman DL, Agulnik I, Hancock S, Silver LM, Papaioannou VE. Dev Biol. 1996 Dec 15;180(2):534-42. 10.1006/dbio.1996.0326 PubMed 8954725
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