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Addgene

pSoup 0800 (35S::REN)
(Plasmid #44469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44469 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSoup
  • Backbone size w/o insert (bp) 9275
  • Total vector size (bp) 11674
  • Vector type
    Plant Transformation

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow for 48hrs.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    A Renilla-derived luciferase gene
  • Alt name
    REN
  • Insert Size (bp)
    2399
  • Promoter 35S Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer RAJ-198 (CTTAGTTTACCCGCCAATATATCCTGTCA)
  • 3′ sequencing primer RAJ-199 (AGATCTTGGCAGGATATATTGTGGTGTAAC)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference: Johnson RA, Hellens RP, Love DR (2011) A transient assay for recombination demonstrates that Arabidopsis SNM1 and XRCC3 enhance non-homologous recombination. Genetics and Molecular Research 10 (3):2104-2132. doi:10.4238/vol10-3gmr1347

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSoup 0800 (35S::REN) was a gift from Roger Hellens (Addgene plasmid # 44469)
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