pHEX2
(Plasmid #44559)

• Purpose: (Empty Backbone)
• Depositing Labs: Andrew Gleave, Roger Hellens
• Publication: Hellens et al Plant Methods. 2005 Dec 18;1:13.

Backbone

• Vector backbone: pART27
• Backbone manufacturer: Gleave AP (1992) Plant Mol Biol 20:1203-1207
• Backbone size (bp): 11608
• Modifications to backbone: Gateway conversion of pART27
• Vector type: Plant Transformation Vector
• Promoter: CaMV35S
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13

pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207