gfap:GFP-NUC
(Plasmid #44964)

• Depositing Lab: Pamela Raymond
• Publication: Bernardos et al J Neurosci. 2007 Jun 27;27(26):7028-40.

Backbone

• Vector backbone: pEGFP-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 11500
• Modifications to backbone: NLS ligated onto C-terminus of EGFP via BsrGI and NotI sites
• Vector type: Zebrafish transgenic targeting

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gfap regulatory elements
• Alt name: gfap 5' upstream and intronic regulator elements
• Alt name: gfap
• Alt name: glial fibrillary acid protein
• Species: D. rerio (zebrafish)
• Insert Size (bp): 7300
• Mutation: Contains only Intron1 (+497 to +4876) and 5'/Exon1 (-3000 to +19)
• GenBank ID: Exon 1: AY151288.1
• Entrez Gene: gfap (a.k.a. cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485)
• Promoter: gfap regulatory elements
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBR322ori-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid encodes a nuclear-targeted EGFP reporter under the control of the regulatory elements in the 5' upstream and intronic regions of the glial-specific zebrafish gene glial fibrillary acid protein (gfap). It can be injected into zebrafish embryos to create germline transgenics.