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(Plasmid #44964)


Item Catalog # Description Quantity Price (USD)
Plasmid 44964 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4200
  • Total vector size (bp) 11500
  • Modifications to backbone
    NLS ligated onto C-terminus of EGFP via BsrGI and NotI sites
  • Vector type
    Zebrafish transgenic targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    gfap regulatory elements
  • Alt name
    gfap 5' upstream and intronic regulator elements
  • Alt name
  • Alt name
    glial fibrillary acid protein
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Mutation
    Contains only Intron1 (+497 to +4876) and 5'/Exon1 (-3000 to +19)
  • GenBank ID
    Exon 1: AY151288.1
  • Entrez Gene
    gfap (a.k.a. cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485)
  • Promoter gfap regulatory elements
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid encodes a nuclear-targeted EGFP reporter under the control of the regulatory elements in the 5' upstream and intronic regions of the glial-specific zebrafish gene glial fibrillary acid protein (gfap). It can be injected into zebrafish embryos to create germline transgenics.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gfap:GFP-NUC was a gift from Pamela Raymond (Addgene plasmid # 44964)
  • For your References section:

    Late-stage neuronal progenitors in the retina are radial Muller glia that function as retinal stem cells. Bernardos RL, Barthel LK, Meyers JR, Raymond PA. J Neurosci. 2007 Jun 27;27(26):7028-40. 10.1523/JNEUROSCI.1624-07.2007 PubMed 17596452