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Addgene

pRRLsincPPT-hPGK-mSEAP-WPRE
(Plasmid #45017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45017 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRRLsincPPT-hPGK-mcs-WPRE
  • Backbone manufacturer
    Addgene plasmid 44710
  • Backbone size w/o insert (bp) 6684
  • Total vector size (bp) 8234
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mSEAP
  • Alt name
    mouse secreted embryonic alkaline phosphatase
  • Alt name
    Alppl2
  • Alt name
    alkaline phosphatase, placental-like 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • GenBank ID
    AY054302.1, AAL17657.1; NM_007433.3, NP_031459.3
  • Entrez Gene
    Alppl2 (a.k.a. Akp5, C77216, D1Ertd816e, EAP)
  • Promoter hPGK

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRLsincPPT-hPGK-mSEAP-WPRE was a gift from Didier Trono (Addgene plasmid # 45017)

  • For your References section:

    A KRAB/KAP1-miRNA Cascade Regulates Erythropoiesis Through Stage-Specific Control of Mitophagy. Barde I, Rauwel B, Marin-Florez RM, Corsinotti A, Laurenti E, Verp S, Offner S, Marquis J, Kapopoulou A, Vanicek J, Trono D. Science. 2013 Mar 14. 10.1126/science.1232398 PubMed 23493425
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