Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45390||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5700
- Total vector size (bp) 6900
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsUse AG1 or BL21(DE3)pLysS E. coli for protein overexpression.
SpeciesH. sapiens (human)
Insert Size (bp)1170
/ Fusion Protein
- His (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Nde I (not destroyed)
- 3′ cloning site BamH I (not destroyed)
- 5′ sequencing primer 5'-TAATACGACTCACTATAGGG-3' Novagen
- 3′ sequencing primer 5'-GCTAGTTATTGCTCAGCGG-3' Novagen (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byResource Center and Primary Database of the German Human Genome Project
Terms and Licenses
- Not Available to Industry
Our lab isolated the protein based on it's ability to bind to a region of the PAI-1 (plasminogen activator inhibitor type 1) 3'UTR that we had determined to be involved in the cAMP regulation of mRNA stability. A search of the database revealed that the cDNA for the protein had been cloned by the German Genome Project and dubbed hypothetical protein. They kindly provided us with the clone, which we subcloned into pET-15b for expression of the protein.
Subsequently, we discovered isoforms of the protein with or without 6 and/or 15 amino acid insertions. These variants are now subcloned into pCELF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-15b PAI-RBP was a gift from Thomas Gelehrter (Addgene plasmid # 45390 ; http://n2t.net/addgene:45390 ; RRID:Addgene_45390)
For your References section:Identification and cDNA cloning of a novel RNA-binding protein that interacts with the cyclic nucleotide-responsive sequence in the Type-1 plasminogen activator inhibitor mRNA. Heaton JH, Dlakic WM, Dlakic M, Gelehrter TD. J Biol Chem. 2001 Feb 2;276(5):3341-7. Epub 2000 Sep 22. 10.1074/jbc.M006538200 PubMed 11001948
Map uploaded by the depositor.