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Addgene

c-myc/EGFP
(Plasmid #45990)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45990 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 13600
  • Vector type
    EGFP reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc/EGFP reporter gene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    10700
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site EcoR V (destroyed during cloning)
  • 5′ sequencing primer GTAAA ACGAC GGCCA GT
  • 3′ sequencing primer CAGGA AACAG CTATG AC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    c-myc/EGFP was a gift from Hodaka Fujii (Addgene plasmid # 45990)

  • For your References section:

    Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene. Fujii H. J Biosci Bioeng. 2009 Nov;108(5):438-40. doi: 10.1016/j.jbiosc.2009.04.022. 10.1016/j.jbiosc.2009.04.022 PubMed 19804871
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