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Addgene

pCAG-MCSn1
(Plasmid #45996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45996 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAG
  • Backbone size (bp) 3243
  • Vector type
    Mammalian Expression
  • Promoter chicken beta-actin promoter + CMV enhancer

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCAG-MCSn1 was derived from pDRIVE-CAG (Invivogen) by removing the LacZ cassette and inserting the multiple cloning site (MCS) from pEGFP-N1 (Clontech).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-MCSn1 was a gift from Philipp Mergenthaler (Addgene plasmid # 45996)

  • For your References section:

    Mitochondrial hexokinase II (HKII) and phosphoprotein enriched in astrocytes (PEA15) form a molecular switch governing cellular fate depending on the metabolic state. Mergenthaler P, Kahl A, Kamitz A, van Laak V, Stohlmann K, Thomsen S, Klawitter H, Przesdzing I, Neeb L, Freyer D, Priller J, Collins TJ, Megow D, Dirnagl U, Andrews DW, Meisel A. Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1518-23. doi: 10.1073/pnas.1108225109. Epub 2012 Jan 10. 10.1073/pnas.1108225109 PubMed 22233811
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