|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46013||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerLidstrom Lab (PMID 8021187)
- Backbone size (bp) 6000
Modifications to backboneloxP-flanked gentamicin-resistance cassette (encoded by aaaC1) was amplified from pLoxGen4 (Palmeros, 2000), using CM-ufkMCS and CM-dfkMCS (see Addgene plasmid 46012 for sequences), and cloned into pCR2.1 (Invitrogen) to produce pCM350. The 1-kb AatII/SacI fragment from pCM350 was cloned between the AatII and SacI sites of pCM184 (Addgene plasmid 46012) to generate pCM351.
- Promoter na
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
This allelic exchange vector is used with Cre expression vectors (Addgene plasmids 45863 or 45864) to generate unmarked mutants in a broad bacterial host range.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCM351 was a gift from Mary Lidstrom (Addgene plasmid # 46013 ; http://n2t.net/addgene:46013 ; RRID:Addgene_46013)
For your References section:Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Marx CJ, Lidstrom ME. Biotechniques. 2002 Nov;33(5):1062-7. 10.2144/02335rr01 PubMed 12449384