Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24 - January 1, 2020. For more information, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCM351
(Plasmid #46013)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46013 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAYC61
  • Backbone manufacturer
    Lidstrom Lab (PMID 8021187)
  • Backbone size (bp) 6000
  • Modifications to backbone
    loxP-flanked gentamicin-resistance cassette (encoded by aaaC1) was amplified from pLoxGen4 (Palmeros, 2000), using CM-ufkMCS and CM-dfkMCS (see Addgene plasmid 46012 for sequences), and cloned into pCR2.1 (Invitrogen) to produce pCM350. The 1-kb AatII/SacI fragment from pCM350 was cloned between the AatII and SacI sites of pCM184 (Addgene plasmid 46012) to generate pCM351.
  • Vector type
    Cre/Lox
  • Promoter na

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

This allelic exchange vector is used with Cre expression vectors (Addgene plasmids 45863 or 45864) to generate unmarked mutants in a broad bacterial host range.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM351 was a gift from Mary Lidstrom (Addgene plasmid # 46013 ; http://n2t.net/addgene:46013 ; RRID:Addgene_46013)
  • For your References section:

    Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Marx CJ, Lidstrom ME. Biotechniques. 2002 Nov;33(5):1062-7. 10.2144/02335rr01 PubMed 12449384