|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46138||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS+
- Total vector size (bp) 6131
Vector typeCre/Lox ; mutagenic gene trap for zebrafish
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)3900
- Cloning method Restriction Enzyme
- 5′ cloning site DraIII (unknown if destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer mCherryR
- 3′ sequencing primer M13 F20 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
mCherry is in the "even" or "0" reading frame starting from the exon.
This is a Tol2-based FlExTrap vector. It consists of a highly mutagenic gene trap contained within the FlEx cassette that can be stably inverted by both Cre and Flp recombinases. FRT/F3 and loxP/lox5171 are incompatible recombination sites used for the Flp and Cre recombinases, respectively, to stably invert the cassette. The reporter gene, mCherry, has its own initiation codon.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFT1.0 was a gift from Wenbiao Chen (Addgene plasmid # 46138 ; http://n2t.net/addgene:46138 ; RRID:Addgene_46138)
For your References section:Conditional control of gene function by an invertible gene trap in zebrafish. Ni TT, Lu J, Zhu M, Maddison LA, Boyd KL, Huskey L, Ju B, Hesselson D, Zhong TP, Page-McCaw PS, Stainier DY, Chen W. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 Aug 20. 10.1073/pnas.1206131109 PubMed 22908272