Purposebacterial expression of GST-myc-Notch1 IC
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47612||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNotch1 intracellular domain
Alt nameNotch1 IC
SpeciesM. musculus (mouse)
Insert Size (bp)2700
Mutationcontains amino acids 1753-2531
Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mi, Mis6, N, N1, Ta, Tan1, lin, lin-12)
- Promoter tac
/ Fusion Proteins
- GST (N terminal on backbone)
- myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Construct also called GST-1111.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST-N1IC was a gift from Urban Lendahl (Addgene plasmid # 47612 ; http://n2t.net/addgene:47612 ; RRID:Addgene_47612)
For your References section:The notch 3 intracellular domain represses notch 1-mediated activation through Hairy/Enhancer of split (HES) promoters. Beatus P, Lundkvist J, Oberg C, Lendahl U. Development. 1999 Sep;126(17):3925-35. PubMed 10433920
Map uploaded by the depositor.