CMV/N3IC-HA
(Plasmid #47618)

• Purpose: in vitro transcription and translation of HA-tagged Notch3 Intracellular domain
• Depositing Lab: Urban Lendahl
• Publication: Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

Backbone

• Vector backbone: pCMX
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression ; in vitro transcription and translation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Notch3 Intracellular domain
• Alt name: Notch3
• Alt name: Notch 3
• Species: M. musculus (mouse)
• Mutation: codons 1664 (Met) to 2318 (Ala)
• Entrez Gene: Notch3 (a.k.a. N3, hpbk)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry