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Cdc6-­E2F-­luc
(Plasmid #49105)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49105 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cdc6 mutated promoter
  • Alt name
    cell division cycle 6
  • Alt name
    Cdc6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Mutation
    contains region -1700 to +7; mutations at −43 to −36 and −8 to −1 relative to the transcriptional start site. Specifically, the sequence motifs TTTGGCGG and TTTGGCGC were converted to AATTCCGG and AAAGCTTG, respectively.
  • Entrez Gene
    CDC6 (a.k.a. CDC18L, HsCDC18, HsCDC6, MGORS5)
  • Promoter Cdc6 mutated promoter
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cdc6-­E2F-­luc was a gift from James DeGregori & Joseph Nevins (Addgene plasmid # 49105)

  • For your References section:

    Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Yan Z, DeGregori J, Shohet R, Leone G, Stillman B, Nevins JR, Williams RS. Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3603-8. 10.1073/pnas.95.7.3603 PubMed 9520412
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