GABA(A) receptor subunit a2SE
(Plasmid #49169)

• Purpose: pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
• Depositing Labs: Tija Jacob, Stephen Moss
• Depositing Organization: University College London
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. doi: 10.1523/JNEUROSCI.5050-07.2008.
• Citations: 8 Articles

Backbone

• Vector backbone: modified pRK5
• Backbone manufacturer: BD PharMingen
• Backbone size w/o insert (bp): 5084
• Modifications to backbone: region 5’ of the cloning site is slightly different
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GABA(A) receptor subunit alpha-2
• Alt name: GBRA2
• Species: H. sapiens (human)
• Mutation: T343A
• GenBank ID:
• Entrez Gene: GABRA2 (a.k.a. DEE78, EIEE78)
• Promoter: CMV
• Tags / Fusion Proteins:
  • bovine alpha 1 signal sequence (N terminal on insert)
  • HA (N terminal on insert)
  • pHGFP (pHIuorin) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: P5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 49169-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 49169-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.