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D588 lys2::HIS3 Disruptor Converter
(Plasmid #51675)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51675 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HIS3
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HIS3 (a.k.a. YOR202W, HIS10, HIS8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Plasmid D588 (lys2::HIS3 ) was constructed in two steps. First, plasmid YDp-K was cut with BglII and XhoI and ligated, deleting a 2477 bp fragment inside the LYS2 gene. This plasmid was then cleaved with BglII and a 1.2 kb BamHI fragment from YDp-H containing HIS3 was inserted, creating D558.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D588 lys2::HIS3 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51675 ; http://n2t.net/addgene:51675 ; RRID:Addgene_51675)
  • For your References section:

    New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713