D588 lys2::HIS3 Disruptor Converter
(Plasmid #51675)

• Purpose: Yeast marker swap plasmid for converting LYS2 to HIS3
• Depositing Lab: David Stillman
• Publication: Voth et al Yeast. 2003 Aug;20(11):985-93.

Backbone

• Vector backbone: YDp-K
• Vector type: Yeast Expression ; yeast marker swap
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HIS3
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: HIS3 (a.k.a. YOR202W, HIS10, HIS8)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid D588 (lys2::HIS3 ) was constructed in two steps. First, plasmid YDp-K was cut with BglII and XhoI and ligated, deleting a 2477 bp fragment inside the LYS2 gene. This plasmid was then cleaved with BglII and a 1.2 kb BamHI fragment from YDp-H containing HIS3 was inserted, creating D558.