D771 leu2::LYS2 Disruptor Converter
PurposeYeast marker swap plasmid for converting LEU2 to LYS2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51677||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression ; yeast marker swap
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesS. cerevisiae (budding yeast)
Entrez GeneLYS2 (a.k.a. YBR115C)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid D771 (leu2::LYS2) was constructed in two steps. First, plasmid YDp-K was cut with SalI and an EcoRI linker was inserted, constructing plasmid D764. Next, plasmid pIC19H–SXL2 was cut with EcoRI and a 5 kb EcoRI fragment containing LYS2 from plasmid D764 was inserted, creating D771.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:D771 leu2::LYS2 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51677 ; http://n2t.net/addgene:51677 ; RRID:Addgene_51677)
For your References section:New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713