|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54125||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt nameIntraflagellar transport 88
SpeciesM. musculus (mouse)
MutationN241D in IFT88
- Promoter CMV
/ Fusion Protein
- mEmerald (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Intraflagellar transport 88. Excitation = 487; Emission = 509. Localization data: Cilium
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-IFT88-N-18 was a gift from Michael Davidson (Addgene plasmid # 54125 ; http://n2t.net/addgene:54125 ; RRID:Addgene_54125)