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Addgene

pENTR-H1
(Plasmid #55819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55819 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pENTR-1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 2500
  • Modifications to backbone
    introduction of H1 promoter
  • Vector type
    Mammalian Expression, RNAi ; GATEWAY
  • Promoter H1 RNA gene

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer 5'-CGGATTTGAACGTTGTGAAG-3' (ON2933)
  • 3′ sequencing primer 5'-GTAACATCAGAGATTTTGAGACAC-3' (ON972)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H1 promoter cloned from plasmid pRETRO-SUPER (Reuven Agami)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-H1 was a gift from Stephan Geley (Addgene plasmid # 55819)

  • For your References section:

    Development of a Multipurpose GATEWAY-Based Lentiviral Tetracycline-Regulated Conditional RNAi System (GLTR). Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S. PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014. 10.1371/journal.pone.0097764 PubMed 24841113
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