pENTR-H1
(Plasmid
#55819)
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Purpose(Empty Backbone) GATEWAY cloning compatible shRNA expression vector; H1 RNA gene promoter; Kanamycin resistance
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 55819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepENTR-1A
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Backbone manufacturerInvitrogen
- Backbone size (bp) 2500
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Modifications to backboneintroduction of H1 promoter
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Vector typeMammalian Expression, RNAi ; GATEWAY
- Promoter H1 RNA gene
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer 5'-CGGATTTGAACGTTGTGAAG-3' (ON2933)
- 3′ sequencing primer 5'-GTAACATCAGAGATTTTGAGACAC-3' (ON972) (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byH1 promoter cloned from plasmid pRETRO-SUPER (Reuven Agami)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-H1 was a gift from Stephan Geley (Addgene plasmid # 55819) -
For your References section:
Development of a Multipurpose GATEWAY-Based Lentiviral Tetracycline-Regulated Conditional RNAi System (GLTR). Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S. PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014. 10.1371/journal.pone.0097764 PubMed 24841113