pENTR-H1
(Plasmid #55819)

• Purpose: (Empty Backbone) GATEWAY cloning compatible shRNA expression vector; H1 RNA gene promoter; Kanamycin resistance
• Depositing Lab: Stephan Geley
• Publication: Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	55819	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Discontinued

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pENTR-1A
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 2500
• Modifications to backbone: introduction of H1 promoter
• Vector type: Mammalian Expression, RNAi ; GATEWAY
• Promoter: H1 RNA gene

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: 5'-CGGATTTGAACGTTGTGAAG-3' (ON2933)
• 3′ sequencing primer: 5'-GTAACATCAGAGATTTTGAGACAC-3' (ON972)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H1 promoter cloned from plasmid pRETRO-SUPER (Reuven Agami)
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry