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pCAG-HA-NLGN3 R451C
(Plasmid #59319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59319 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 59319-D50 50 μg of DNA in Tris buffer $495
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DNA 59319-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4835
  • Total vector size (bp) 7313
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLGN3 R451C
  • Alt name
    Neuroligin 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2478
  • Mutation
    Arginine 451 to Cysteine (R451C mutation)
  • GenBank ID
    NM_018977 BC051715
  • Entrez Gene
    NLGN3 (a.k.a. HNL3)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / XhoI).
HA-epitope tag was introduced at the mature N-terminus after the signal sequence

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-NLGN3 R451C was a gift from Peter Scheiffele (Addgene plasmid # 59319 ; http://n2t.net/addgene:59319 ; RRID:Addgene_59319)

  • For your References section:

    Disorder-associated mutations lead to functional inactivation of neuroligins. Chih B, Afridi SK, Clark L, Scheiffele P. Hum Mol Genet. 2004 Jul 15;13(14):1471-7. Epub 2004 May 18. 10.1093/hmg/ddh158 PubMed 15150161
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