pENTR-D-TOPO-PAmCherry1-MCS
(Plasmid #60608)

• Purpose: Cloning PAmCherry1
• Depositing Labs: Steven Chu, Xiaolin Nan
• Publication: Nan et al Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18519-24. doi: 10.1073/pnas.1318188110. Epub 2013 Oct 24.

Backbone

• Vector backbone: pENTR/D-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2580
• Total vector size (bp): 3357
• Modifications to backbone: Added flanking MCSs
• Vector type: Cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAmCherry1
• Alt name: Photoactivatable mCherry
• Insert Size (bp): 708

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • GenBank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was used in Nickerson et al., 2014 (PMID: 24963703) for generation of PAmCherry1 fragments (addgene.org/60545 and addgene.org/60546) for BiFC-PALM.