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NKP66 pEGFP-N2 (1176)
(Plasmid #62039)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62039 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MARCHV
  • Alt name
    NKP66
  • Species
    H. sapiens (human)
  • Entrez Gene
    MARCH5 (a.k.a. RP11-543N17.3, MARCH-V, MITOL, RNF153)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NKP66 pEGFP-N2 (1176) was a gift from Eric Schirmer (Addgene plasmid # 62039 ; http://n2t.net/addgene:62039 ; RRID:Addgene_62039)
  • For your References section:

    A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4/Tmem53 as involved in stress-dependent cell cycle withdrawal. Korfali N, Srsen V, Waterfall M, Batrakou DG, Pekovic V, Hutchison CJ, Schirmer EC. PLoS One. 2011 Apr 14;6(4):e18762. doi: 10.1371/journal.pone.0018762. 10.1371/journal.pone.0018762 PubMed 21533191