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SP-Cas9
(Plasmid #62731)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62731 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-15
  • Backbone size w/o insert (bp) 5562
  • Total vector size (bp) 9822
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SP-CAS9
  • Alt name
    CAS9 from Streptococcus pyogenes serotype M1
  • Species
    Streptococcus pyogenes serotype M1
  • Insert Size (bp)
    4179
  • Mutation
    Codon optimazed for e.coli
  • GenBank ID
    GI:81533697 Q99ZW2
  • Promoter T7
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • His tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GATCGAGATCTCGATCCCGCG
  • 3′ sequencing primer TTTCAGCAAAAAACCCCTCAAGACC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SP-Cas9 was a gift from Niels Geijsen (Addgene plasmid # 62731 ; http://n2t.net/addgene:62731 ; RRID:Addgene_62731)
  • For your References section:

    Efficient Intracellular Delivery of Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V, Lebbink RJ, Rehmann H, Geijsen N. Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028. 10.1016/j.cell.2015.03.028 PubMed 25910214