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pMSCVpuro-PCAF
(Plasmid #63705)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63705 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSCVpuro
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 8800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCAF
  • Alt name
    KAT2B
  • Alt name
    P300/CBP-associated factor
  • Alt name
    K(lysine) acetyltransferase 2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • GenBank ID
    BC060823 BC060823
  • Entrez Gene
    KAT2B (a.k.a. CAF, P/CAF, PCAF)
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer MSCV5
  • 3′ sequencing primer MSCV3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCVpuro-PCAF was a gift from Kai Ge (Addgene plasmid # 63705 ; http://n2t.net/addgene:63705 ; RRID:Addgene_63705)
  • For your References section:

    Gcn5 and PCAF negatively regulate interferon-beta production through HAT-independent inhibition of TBK1. Jin Q, Zhuang L, Lai B, Wang C, Li W, Dolan B, Lu Y, Wang Z, Zhao K, Peng W, Dent SY, Ge K. EMBO Rep. 2014 Nov;15(11):1192-201. doi: 10.15252/embr.201438990. Epub 2014 Sep 30. 10.15252/embr.201438990 PubMed 25269644