pMSCVpuro-Gcn5
(Plasmid #63706)

• Purpose: retroviral expression of mouse Gcn5 in mammalian cells
• Depositing Lab: Kai Ge
• Publication: Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi: 10.15252/embr.201438990. Epub 2014 Sep 30.

Backbone

• Vector backbone: pMSCVpuro
• Backbone size w/o insert (bp): 6300
• Total vector size (bp): 8800
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gcn5
• Alt name: Kat2a
• Alt name: Gcn5l2
• Species: M. musculus (mouse)
• Insert Size (bp): 2500
• GenBank ID: NM_020004.5
• Entrez Gene: Kat2a (a.k.a. 1110051E14Rik, Gcn5, Gcn5l2, mmGCN5)
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: MSCV5
• 3′ sequencing primer: MSCV3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Subcloned from Addgene plasmid #23098 provided by Sharon Dent, MD Anderson Cancer Center

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry