pNTAP-PAK1 WT
(Plasmid
#64826)
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Purposeexpression of calmodulin and streptavidin-binding peptides tagged to PAK1 WT
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64826 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNTAP-A
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Backbone manufacturerAgilent Technologies
- Backbone size w/o insert (bp) 4530
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePAK1
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Alt namep21 protein (Cdc42/Rac)-activated kinase 1
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SpeciesH. sapiens (human)
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GenBank ID5058
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Entrez GenePAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
- Promoter CMV
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Tags
/ Fusion Proteins
- CBP (N terminal on backbone)
- SBP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNTAP-PAK1 WT was a gift from Maria Diakonova (Addgene plasmid # 64826)