PurposeExpression of dominant negative Axl in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65223||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5900
- Total vector size (bp) 7400
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert nameAXL DN
SpeciesH. sapiens (human)
Insert Size (bp)1500
Mutationdeleted cytoplasmic domain
Entrez GeneAXL (a.k.a. ARK, JTK11, Tyro7, UFO)
- Promoter LTR
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site FspI (destroyed during cloning)
- 5′ sequencing primer CTCTCCCCCTTGAACCTC
- 3′ sequencing primer GACTTTCCACACCCTAACTG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Insert contains extracellular and transmembrane domains of AXL.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLXSN-Axl DN was a gift from Axel Ullrich (Addgene plasmid # 65223 ; http://n2t.net/addgene:65223 ; RRID:Addgene_65223)
For your References section:AXL is a potential target for therapeutic intervention in breast cancer progression. Zhang YX, Knyazev PG, Cheburkin YV, Sharma K, Knyazev YP, Orfi L, Szabadkai I, Daub H, Keri G, Ullrich A. Cancer Res. 2008 Mar 15;68(6):1905-15. doi: 10.1158/0008-5472.CAN-07-2661. 10.1158/0008-5472.CAN-07-2661 PubMed 18339872