gfap-EGFP donor
(Plasmid #65564)

• Purpose: Intron targeting-mediated EGFP knockin at the zebrafish gfap locus
• Depositing Lab: Jiu-lin Du
• Publication: Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.

Backbone

• Vector backbone: PMD-19-T
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 2692
• Total vector size (bp): 5429
• Vector type: zebrafish injection

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gfap-EGFP
• Alt name: gfap (left arm)-EGFP-gfap (right arm)
• Species: D. rerio (zebrafish)
• Entrez Gene: gfap (a.k.a. cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485)
• Promoter: none

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • gfap-EGFP donor

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

P2A sequence was replaced with a flexible serine-serine linker sequence (TCTTCT)