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pT2-7xTcf-NLS-YNL-CP
(Plasmid #65714)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65714 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 65714-D50 50 μg of DNA in Tris buffer $495
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DNA 65714-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBluescript II SK(+) based Tol2 donor vector
  • Backbone manufacturer
    Agilent Biotechnology, National Institute of Genetics (Dr Koichi Kawakami Lab)
  • Backbone size w/o insert (bp) 7700
  • Total vector size (bp) 9900
  • Vector type
    Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
  • Alt name
    7xTcf-NLS-YNL-CP
  • Species
    Synthetic
  • Insert Size (bp)
    2200

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GGATGGGGCTCTAGGAATAAAATATC
  • 3′ sequencing primer GCTCCAGACTGCCTTGGGAAAAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Nano-lantern/pcDNA3 (gifted from Dr Takeharu Nagai), Tol2 system (gifted from Dr Koichi Kawakami)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2-7xTcf-NLS-YNL-CP was a gift from Yasushi Okada (Addgene plasmid # 65714 ; http://n2t.net/addgene:65714 ; RRID:Addgene_65714)

  • For your References section:

    Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y, Nagai T. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23. 10.1073/pnas.1418468112 PubMed 25831507
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