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Addgene

alpha-actinin SR12-GFP
(Plasmid #66934)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66934 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Alpha-actinin 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    containing only the first 29 amino acids of the protein, domains SR1 and SR2 (amino acids 267–507), and the last 5 amino acids of the protein.
  • Entrez Gene
    ACTN1 (a.k.a. BDPLT15)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site unknown (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha-actinin SR12-GFP was a gift from Pere Roca-Cusachs & Michael Sheetz (Addgene plasmid # 66934)

  • For your References section:

    Integrin-dependent force transmission to the extracellular matrix by alpha-actinin triggers adhesion maturation. Roca-Cusachs P, del Rio A, Puklin-Faucher E, Gauthier NC, Biais N, Sheetz MP. Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):E1361-70. doi: 10.1073/pnas.1220723110. Epub 2013 Mar 20. 10.1073/pnas.1220723110 PubMed 23515331
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