mRFP-dmBACCS2
(Plasmid
#67630)
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PurposeDrosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAc5.1/V5-His A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5362
- Total vector size (bp) 7643
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Vector typeDrosophila expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemRFP-dmBACCS2
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SpeciesD. melanogaster (fly); Avena sativa (oat)
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Insert Size (bp)2281
- Promoter Drosophila actin gene 5C gene promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer AC5
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mRFP-dmBACCS2 was a gift from Takao Nakata (Addgene plasmid # 67630 ; http://n2t.net/addgene:67630 ; RRID:Addgene_67630) -
For your References section:
Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T. Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021. 10.1038/ncomms9021 PubMed 26282514
