Purpose(Empty Backbone) Derivative of pKDH plant gene expression vector containing the M24 promoter
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67885||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerStratagene, fragments of the vector were synthesized by GenArt-Life Technologies
- Backbone size (bp) 6675
Modifications to backboneM24 promoter inserted into EcoRI, HindIII restriction sites
Vector typePlant Expression
- Promoter M24 cloned into EcoRI and HindIII sites of pKDH
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
- 3′ sequencing primer NOSterm-R (5'-attgccaaatgtttgaacga-3') (Common Sequencing Primers)
The difference between this plasmid and pSIM24 KanR from the same deposit is that pKDH has a hygromycin resistance cassette whereas pSIM24 KanR has a kanamycin resistance cassette. Both kanamycin and hygromycin are used for selection in plants.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKDHM24 was a gift from Indu Maiti (Addgene plasmid # 67885)
For your References section:pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. Sahoo DK, Dey N, Maiti IB. PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014. PONE-D-14-03371 [pii] PubMed 24897541