pKDHM24
(Plasmid #67885)

• Purpose: (Empty Backbone) Derivative of pKDH plant gene expression vector containing the M24 promoter
• Depositing Lab: Indu Maiti
• Publication: Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014.

Backbone

• Vector backbone: N/A
• Backbone manufacturer: Stratagene, fragments of the vector were synthesized by GenArt-Life Technologies
• Backbone size (bp): 6675
• Modifications to backbone: M24 promoter inserted into EcoRI, HindIII restriction sites
• Vector type: Plant Expression
• Promoter: M24 cloned into EcoRI and HindIII sites of pKDH
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
• 3′ sequencing primer: NOSterm-R (5'-attgccaaatgtttgaacga-3')

Resource Information

• Supplemental Documents:
  • pKDH vector backbone genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The difference between this plasmid and pSIM24 KanR from the same deposit is that pKDH has a hygromycin resistance cassette whereas pSIM24 KanR has a kanamycin resistance cassette. Both kanamycin and hygromycin are used for selection in plants.